gibson assembly master mix (New England Biolabs)
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gibson assembly master mix
Gibson Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly/Gibson+Assembly+Master+Mix/pmc12967149-217-24-28
Average 99 stars, based on 6210 article reviews
Gibson Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly/Gibson+Assembly+Master+Mix/pmc12967149-217-24-28
Average 99 stars, based on 6210 article reviews
gibson assembly master mix - by Bioz Stars,
2026-09
99/100 stars
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Cloning:Article Title: Genomic heterogeneity of NAD(P)H dehydrogenase predisposes Cryptosporidium to clofazimine resistance. Article Snippet: .. Guide oligo nucleotides (Sigma-Aldrich) were introduced into the C. parvum Cas9/U6 plasmid29 by restriction cloning (see ref. 72 for guide design) and repair templates were constructed by Article Title: Metabolic engineering of Methanothermobacter thermautotrophicus ΔH for recombinant acetoin production Article Snippet: DNA fragments for cloning were generated via PCR using the Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), according to the manufacturer's instructions and subsequently purified using the QIAquick® PCR Purification Kit (Qiagen GmbH, Hilden, Germany). .. Cloning was performed via Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: .. The PCR product was then ligated into the linearized vector by Construct:Article Title: Genomic heterogeneity of NAD(P)H dehydrogenase predisposes Cryptosporidium to clofazimine resistance. Article Snippet: .. Guide oligo nucleotides (Sigma-Aldrich) were introduced into the C. parvum Cas9/U6 plasmid29 by restriction cloning (see ref. 72 for guide design) and repair templates were constructed by Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Plasmid Preparation:Article Title: Metabolic engineering of Methanothermobacter thermautotrophicus ΔH for recombinant acetoin production Article Snippet: .. Resulting DNA fragments were merged via Article Title: Divergent CRD-Dependent Mechanisms Govern RAS Isoform-Selective Recruitment of CRAF and ARAF Article Snippet: NanoBiT® CMV MCS BiBiT vector, which contains a BRAF N- terminal fused with LgBiT and CRAF N-terminal fused with SmBiT, were purchased from Promega. .. KRAS LgBiT and CRAF SmBiT was generated using the standard Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Article Title: Metabolic engineering of Methanothermobacter thermautotrophicus ΔH for recombinant acetoin production Article Snippet: This tetracycline regulatory cassette was flanked by Asc I and Spe I restriction sites, codon-optimized for M. thermautotrophicus , and finally synthesized as a gBlock (Integrated DNA Technologies, Coralville, IA, USA; ). .. The gBlock was subcloned into the pMiniT 2.0 vector and subsequently ligated into pMVS1111a_P hmtB _bgaB via Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: .. The PCR product was then ligated into the linearized vector by Generated:Article Title: Divergent CRD-Dependent Mechanisms Govern RAS Isoform-Selective Recruitment of CRAF and ARAF Article Snippet: NanoBiT® CMV MCS BiBiT vector, which contains a BRAF N- terminal fused with LgBiT and CRAF N-terminal fused with SmBiT, were purchased from Promega. .. KRAS LgBiT and CRAF SmBiT was generated using the standard Expressing:Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Sequencing:Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Amplification:Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation Article Snippet: The two DNA halves were amplified separately using Phusion polymerase (NEB #M0530L) followed by an overlap extension polymerase chain reaction (PCR) with a forward primer containing a 5′ T7 promoter overhang. .. The amplified products were cloned into pcDNA3.1 using Clone Assay:Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation Article Snippet: The two DNA halves were amplified separately using Phusion polymerase (NEB #M0530L) followed by an overlap extension polymerase chain reaction (PCR) with a forward primer containing a 5′ T7 promoter overhang. .. The amplified products were cloned into pcDNA3.1 using Polymerase Chain Reaction:Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation Article Snippet: .. The PCR product was then ligated into the linearized vector by |